foxp1 promoter methylation analysis Search Results


gene exp foxp1 hs00908900 m1  (Thermo Fisher)
87
Thermo Fisher gene exp foxp1 hs00908900 m1
Differential DNA methylation and expression ( q < 0.05) in islets from 25 T2D cases vs. 75 controls overlap with islet-specific markers of transcriptional regulation , , open chromatin regions (OCRs) , and differentially methylated regions (DMRs) identified by whole-genome bisulfite sequencing (WGBS) of human islets . a <t>FOXP1</t> has 30 differentially methylated sites and reduced expression in islets from T2D cases vs. controls. This region overlaps with markers of active enhancers (class I-III; EI-EIII) and promoters (P), OCRs, and five islet-specific transcription factors (TFs). WGBS previously found 10 DMRs in FOXP1 in islets from T2D cases vs. controls. b TBC1D4 shows increased DNA methylation and decreased expression in islets from T2D cases vs. controls. This gene further displays 20 markers of the islet regulome, eight T2D-associated DMRs, 17 OCRs, and several binding regions for islet-specific TFs. c The TSS of RHOT1 displays an OCR, marks of active promoter and binding sites for three islet-specific TFs. RHOT1 expression was reduced and an intronic site showed reduced DNA methylation in islets from T2D cases vs. controls. d CABLES1 has altered methylation of five sites and reduced expression in islets from T2D cases vs. controls. TSS and the upstream region are in an OCR and have chromatin marks of active enhancer and promoter, and the region has 11 binding sites for islet-specific TFs. Chromosomal positions are based on hg38. Data presented as mean ± SEM. * q < 0.05 for T2D cases ( n = 25) vs. Controls ( n = 75 for DNA methylatio n and 72 for gene expression), based on linear regressions adjusted for age, BMI, sex, days in culture, and islet purity. The p -values were corrected for multiple testing with FDR analysis. Source data are provided as a Source Data file.
Gene Exp Foxp1 Hs00908900 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/foxp1+promoter+methylation+analysis/Gene+Exp%2E+FOXP1%2C+Hs00908900_m1/pmc10716521-404-31-15
Average 87 stars, based on 1 article reviews
gene exp foxp1 hs00908900 m1 - by Bioz Stars, 2026-09
87/100 stars
  Buy from Supplier
t-cell differentiation factor forkhead box p1 [foxp1]  (Epimmune Inc)
90
Epimmune Inc t-cell differentiation factor forkhead box p1 [foxp1]
Differential DNA methylation and expression ( q < 0.05) in islets from 25 T2D cases vs. 75 controls overlap with islet-specific markers of transcriptional regulation , , open chromatin regions (OCRs) , and differentially methylated regions (DMRs) identified by whole-genome bisulfite sequencing (WGBS) of human islets . a <t>FOXP1</t> has 30 differentially methylated sites and reduced expression in islets from T2D cases vs. controls. This region overlaps with markers of active enhancers (class I-III; EI-EIII) and promoters (P), OCRs, and five islet-specific transcription factors (TFs). WGBS previously found 10 DMRs in FOXP1 in islets from T2D cases vs. controls. b TBC1D4 shows increased DNA methylation and decreased expression in islets from T2D cases vs. controls. This gene further displays 20 markers of the islet regulome, eight T2D-associated DMRs, 17 OCRs, and several binding regions for islet-specific TFs. c The TSS of RHOT1 displays an OCR, marks of active promoter and binding sites for three islet-specific TFs. RHOT1 expression was reduced and an intronic site showed reduced DNA methylation in islets from T2D cases vs. controls. d CABLES1 has altered methylation of five sites and reduced expression in islets from T2D cases vs. controls. TSS and the upstream region are in an OCR and have chromatin marks of active enhancer and promoter, and the region has 11 binding sites for islet-specific TFs. Chromosomal positions are based on hg38. Data presented as mean ± SEM. * q < 0.05 for T2D cases ( n = 25) vs. Controls ( n = 75 for DNA methylatio n and 72 for gene expression), based on linear regressions adjusted for age, BMI, sex, days in culture, and islet purity. The p -values were corrected for multiple testing with FDR analysis. Source data are provided as a Source Data file.
T Cell Differentiation Factor Forkhead Box P1 [Foxp1], supplied by Epimmune Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/foxp1+promoter+methylation+analysis/epimmune+signature/10__1016_slash_s2213___2600_ascii40_18_ascii41_30284___4-15-31-23
Average 90 stars, based on 1 article reviews
t-cell differentiation factor forkhead box p1 [foxp1] - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Differential DNA methylation and expression ( q < 0.05) in islets from 25 T2D cases vs. 75 controls overlap with islet-specific markers of transcriptional regulation , , open chromatin regions (OCRs) , and differentially methylated regions (DMRs) identified by whole-genome bisulfite sequencing (WGBS) of human islets . a FOXP1 has 30 differentially methylated sites and reduced expression in islets from T2D cases vs. controls. This region overlaps with markers of active enhancers (class I-III; EI-EIII) and promoters (P), OCRs, and five islet-specific transcription factors (TFs). WGBS previously found 10 DMRs in FOXP1 in islets from T2D cases vs. controls. b TBC1D4 shows increased DNA methylation and decreased expression in islets from T2D cases vs. controls. This gene further displays 20 markers of the islet regulome, eight T2D-associated DMRs, 17 OCRs, and several binding regions for islet-specific TFs. c The TSS of RHOT1 displays an OCR, marks of active promoter and binding sites for three islet-specific TFs. RHOT1 expression was reduced and an intronic site showed reduced DNA methylation in islets from T2D cases vs. controls. d CABLES1 has altered methylation of five sites and reduced expression in islets from T2D cases vs. controls. TSS and the upstream region are in an OCR and have chromatin marks of active enhancer and promoter, and the region has 11 binding sites for islet-specific TFs. Chromosomal positions are based on hg38. Data presented as mean ± SEM. * q < 0.05 for T2D cases ( n = 25) vs. Controls ( n = 75 for DNA methylatio n and 72 for gene expression), based on linear regressions adjusted for age, BMI, sex, days in culture, and islet purity. The p -values were corrected for multiple testing with FDR analysis. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Genes with epigenetic alterations in human pancreatic islets impact mitochondrial function, insulin secretion, and type 2 diabetes

doi: 10.1038/s41467-023-43719-9

Figure Lengend Snippet: Differential DNA methylation and expression ( q < 0.05) in islets from 25 T2D cases vs. 75 controls overlap with islet-specific markers of transcriptional regulation , , open chromatin regions (OCRs) , and differentially methylated regions (DMRs) identified by whole-genome bisulfite sequencing (WGBS) of human islets . a FOXP1 has 30 differentially methylated sites and reduced expression in islets from T2D cases vs. controls. This region overlaps with markers of active enhancers (class I-III; EI-EIII) and promoters (P), OCRs, and five islet-specific transcription factors (TFs). WGBS previously found 10 DMRs in FOXP1 in islets from T2D cases vs. controls. b TBC1D4 shows increased DNA methylation and decreased expression in islets from T2D cases vs. controls. This gene further displays 20 markers of the islet regulome, eight T2D-associated DMRs, 17 OCRs, and several binding regions for islet-specific TFs. c The TSS of RHOT1 displays an OCR, marks of active promoter and binding sites for three islet-specific TFs. RHOT1 expression was reduced and an intronic site showed reduced DNA methylation in islets from T2D cases vs. controls. d CABLES1 has altered methylation of five sites and reduced expression in islets from T2D cases vs. controls. TSS and the upstream region are in an OCR and have chromatin marks of active enhancer and promoter, and the region has 11 binding sites for islet-specific TFs. Chromosomal positions are based on hg38. Data presented as mean ± SEM. * q < 0.05 for T2D cases ( n = 25) vs. Controls ( n = 75 for DNA methylatio n and 72 for gene expression), based on linear regressions adjusted for age, BMI, sex, days in culture, and islet purity. The p -values were corrected for multiple testing with FDR analysis. Source data are provided as a Source Data file.

Article Snippet: All functional experiments were performed 72 h after the first transfection. siRNA and TaqMan assays (ThermoFisher Scientific, Waltham, MA, USA) targeting CABLES1 (siRNA ID s40774, TaqMan assay ID Hs01106667_m1), FOXP1 (s532467, Hs00908900_m1), RHOT1 (s30650, Hs00430256_m1), or TBC1D4 (s19142, Hs00952765_m1) mRNA were used.

Techniques: DNA Methylation Assay, Expressing, Methylation, Methylation Sequencing, Binding Assay, Gene Expression

a Among methylation sites associated with T2D ( q < 0.05), 10% are located in islet-specific transcription factor (TF) binding regions ( n = 3113) a n d 37% in the islet regulome ( n = 11,857) . b Venn diagram showing T2D-associated methylation sites in regions bound by one or more islet-specific TFs . c Regions bound by islet-specific TFs that overlap differentially methylated sites in the Islet T2D case-control cohort ( q < 0.05). d T2D-associated methylation sites from this study ( n = 31,806) located in regions important for transcriptional regulation in human islets. The islet regulome is based on experimental data in human islets . e Expression after silencing FOXP1, TBC1D4, RHOT1 , and CABLES1 in human islets using siRNA. * p < 0.05 vs. siNC based on a two-sided paired t -test. p = 0.042 (si FOXP1 ), p = 0.002 (si TBC1D4 ), p = 0.003 (si RHOT1 ) and p = 0.002 (si CABLES1 ). f Insulin secretion after silencing of FOXP1, TBC1D4, RHOT1 , and CABLES1 in human islets. # p < 0.05 vs. siNC at 2.8 mM glucose and * p < 0.05 vs. siNC at 16.7 mM glucose based on a two-sided paired t -test. p = 0.011 (si FOXP1 at 16.7 mM), p = 0.025 and p = 0.013 (si TBC1D4 at 2.8 and 16.7 mM, respectively), p = 0.016 and p = 0.030 (si RHOT1 at 2.8 and 16.7 mM, respectively) and p = 0.013 (si CABLES1 at 16.7 mM). g Global expression in human islets silenced for FOXP1 , TBC1D4 , RHOT1 , or CABLES1 using siRNA detected seven genes affected by all four siRNAs. * p < 0.05 vs. siNC based on limma moderated t -test (paired, two-sided), exact p -values in Supplementary Data . h Silencing of RHOT1 increased expression of PINK1 , TFEB , and FIS1 , whereas OPA1 was downregulated. * p < 0.05 based on limma moderated two-sided paired t -test (exact p -values in Supplementary Data ). Data in e – h are shown as mean ± SEM, n = 5 independent experiments in different islet donors. Source data are provided as a Source Data file. siNC non-target control.

Journal: Nature Communications

Article Title: Genes with epigenetic alterations in human pancreatic islets impact mitochondrial function, insulin secretion, and type 2 diabetes

doi: 10.1038/s41467-023-43719-9

Figure Lengend Snippet: a Among methylation sites associated with T2D ( q < 0.05), 10% are located in islet-specific transcription factor (TF) binding regions ( n = 3113) a n d 37% in the islet regulome ( n = 11,857) . b Venn diagram showing T2D-associated methylation sites in regions bound by one or more islet-specific TFs . c Regions bound by islet-specific TFs that overlap differentially methylated sites in the Islet T2D case-control cohort ( q < 0.05). d T2D-associated methylation sites from this study ( n = 31,806) located in regions important for transcriptional regulation in human islets. The islet regulome is based on experimental data in human islets . e Expression after silencing FOXP1, TBC1D4, RHOT1 , and CABLES1 in human islets using siRNA. * p < 0.05 vs. siNC based on a two-sided paired t -test. p = 0.042 (si FOXP1 ), p = 0.002 (si TBC1D4 ), p = 0.003 (si RHOT1 ) and p = 0.002 (si CABLES1 ). f Insulin secretion after silencing of FOXP1, TBC1D4, RHOT1 , and CABLES1 in human islets. # p < 0.05 vs. siNC at 2.8 mM glucose and * p < 0.05 vs. siNC at 16.7 mM glucose based on a two-sided paired t -test. p = 0.011 (si FOXP1 at 16.7 mM), p = 0.025 and p = 0.013 (si TBC1D4 at 2.8 and 16.7 mM, respectively), p = 0.016 and p = 0.030 (si RHOT1 at 2.8 and 16.7 mM, respectively) and p = 0.013 (si CABLES1 at 16.7 mM). g Global expression in human islets silenced for FOXP1 , TBC1D4 , RHOT1 , or CABLES1 using siRNA detected seven genes affected by all four siRNAs. * p < 0.05 vs. siNC based on limma moderated t -test (paired, two-sided), exact p -values in Supplementary Data . h Silencing of RHOT1 increased expression of PINK1 , TFEB , and FIS1 , whereas OPA1 was downregulated. * p < 0.05 based on limma moderated two-sided paired t -test (exact p -values in Supplementary Data ). Data in e – h are shown as mean ± SEM, n = 5 independent experiments in different islet donors. Source data are provided as a Source Data file. siNC non-target control.

Article Snippet: All functional experiments were performed 72 h after the first transfection. siRNA and TaqMan assays (ThermoFisher Scientific, Waltham, MA, USA) targeting CABLES1 (siRNA ID s40774, TaqMan assay ID Hs01106667_m1), FOXP1 (s532467, Hs00908900_m1), RHOT1 (s30650, Hs00430256_m1), or TBC1D4 (s19142, Hs00952765_m1) mRNA were used.

Techniques: Methylation, Binding Assay, Control, Expressing